Journal: bioRxiv

Article Title: Feeder cell – the key component in producing scalable and fit NK cells for therapeutic use

doi: 10.64898/2026.04.21.718880

Figure Lengend Snippet: The NK cells were cryopreserved using five different cryopreservation media at two cell densities, and either with (+) or without (–) centrifugation following thawing. Cells were cultured in NK MACS Expansion Medium, and both cell recovery (expressed as fold change) and viability were assessed immediately upon thawing and three days post-thaw. Fold change in cell number is shown as bars, and viability as overlaid dots. Fold change was calculated relative to the number of NK cells originally frozen per vial, providing an estimate of cell loss during the freeze–thaw cycle and potential recovery during short-term culture. Data are presented as mean ± SD from 3 experiments.

Article Snippet: Thawed NK cells were cultured in NK MACS Expansion Medium consisting of NK MACS basal medium (Miltenyi Biotech, NK MACS medium, cat# 130-112-968) supplemented with 1 % NK MACS supplement (Miltenyi Biotech, NK MACS supplement, cat# 130-113-102), 5 % human AB serum (MP Biomedicals, Human AB Serum, cat# 2930949 or Sigma, Human AB Serum, cat# H5667), 500 IU/mL IL-2 (Miltenyi Biotech, Human IL-2 IS premium grade cat# 130-097-746), 140 IU/ml IL-15 (Miltenyi Biotech, Human IL-15 premium grade, cat# 130-095-765) 50 U/ml penicillin and 50 g/ml streptomycin (Gibco, Penicillin-Streptomycin, cat#15140-122).

Techniques: Centrifugation, Cell Culture, Cell Recovery